N-fixing trees in restoration plantings: effects on nitrogen supply and soil microbial communities

Mixed-species restoration tree plantings are being established increasingly, contributing to mitigate climate change and restore ecosystems. Including nitrogen (N)-fixing tree species may increase carbon (C) sequestration in mixed-species plantings, as these species may substantially increase soil C beneath them. We need to better understand the role of N-fixers in mixed-species plantings to potentially maximize soil C sequestration in these systems. Here, we present a field-based study that asked two specific questions related to the inclusion of N-fixing trees in a mixed-species planting: 1) Do non-N-fixing trees have access to N derived from fixation of atmospheric N2 by neighbouring N-fixing trees? 2) Do soil microbial communities differ under N-fixing trees and non-N-fixing trees in a mixed-species restoration planting? We sampled leaves from the crowns, and litter and soils beneath the crowns of two N-fixing and two non-N-fixing tree species that dominated the planting. Using the 15N natural abundance method, we found indications that fixed atmospheric N was utilized by the non-N-fixing trees, most likely through tight root connections or organic forms of N from the litter layer, rather than through the decomposition of N-fixers litter. While the two N-fixing tree species that were studied appeared to fix atmospheric N, they were substantially different in terms of C and N addition to the soil, as well as microbial community composition beneath them. This shows that the effect of N-fixing tree species on soil carbon sequestration is species-specific, cannot be generalized and requires planting trails to determine if there will be benefits to carbon sequestration. © 2014 Elsevier Ltd.

Wastewater irrigation: the state of play

As demand for fresh water intensifies, wastewater is frequently being seen as a valuable resource. Furthermore, wise reuse of wastewater alleviates concerns attendant with its discharge to the environment. Globally, around 20 million ha of land are irrigated with wastewater, and this is likely to increase markedly during the next few decades as water stress intensifies. In 1995, around 2.3 billion people lived in water-stressed river basins and this could increase to 3.5 billion by 2025. We review the current status of wastewater irrigation by providing an overview of the extent of the practice throughout the world and through synthesizing the current understanding of factors influencing sustainable wastewater irrigation. A theme that emerges is that wastewater irrigation is not only more common in water-stressed regions such as the Near East, but the rationale for the practice also tends to differ between the developing and developed worlds. In developing nations, the prime drivers are livelihood dependence and food security, whereas environmental agendas appear to hold greater sway in the developed world. The following were identified as areas requiring greater understanding for the long-term sustainability of wastewater irrigation: (i) accumulation of bioavailable forms of heavy metals in soils, (ii) environmental fate of organics in wastewater-irrigated soils, (iii) influence of reuse schemes on catchment hydrology, including transport of salt loads, (iv) risk models for helminth infections (pertinent to developing nations), (v) microbiological contamination risks for aquifers and surface waters, (vi) transfer efficiencies of chemical contaminants from soil to plants, (vii) health effects of chronic exposure to chemical contaminants, and (viii) strategies for engaging the public.

Quantifying minimum monolith size and solute dilution from multi-compartment percolation sampler data

Preferential flow affects solute transport in natural soils, leading to high spatiotemporal variation of concentration. A multicompartment solute sampler (MCS), yielding multiple breakthrough curves at a given depth, can monitor tracer movement in a heterogeneous soil. We present a technique to estimate from MCS data whether a soil monolith is sufficiently large to capture preferential flow, which is a necessity for tracer breakthrough curves to be representative. For several soils, we estimate that an MCS should be larger than 0.1 to 0.2 m². We also expand dilution theory to analyze the concentration variations of a tracer passing the control plane monitored by the MCS, in addition to the conventional plume spreading analysis. We characterize the set of locally observed breakthrough curves by the entropy-based dilution index. For given first and second-central moment, the spatially uniform log-normal breakthrough curve maximizes the dilution index. The ratio between observed and maximum dilution index is denoted reactor ratio. For a 300-compartment solute sampler, covering an area of 0.75 m², we compute a reactor ratio of 0.665, compared with 0.04 for stochastic-convective and 1 for convective-dispersive transport. With a single, large collector the reactor ratio would be 0.958, severely underestimating concentration variations. Large collector areas are clearly inadequate to estimate dilution. Values of the dilution index and the reactor ratio for individual sampling compartments indicate efficient longitudinal mixing in most but not all cases, and considerable spatial variation of the leaching process.

Freshwater influences on hydrology and seagrass dynamics of intermittent estuaries

Many temperate estuaries have intermittently open and closed mouths, a feature that is often related to intermittent freshwater input. These systems, often overlooked due to their small size, can have large hydrological variability over medium-term time scales.

This variability presents potential difficulties for estuarine species particularly where anthropogenic alterations to freshwater flows can cause large deviations from natural patterns of tidal influence and inundation of habitat.

Influences of natural and hydrological variability on seagrasses were examined in two central Victorian estuaries with anthropogenically-modified but naturally-intermittent freshwater flows and mouth openings. Comparisons were focused on differences between an estuary with artificially-augmented freshwater inflow and an adjacent system, in which the volume and timing of inflows were altered by a reservoir. Eight additional estuaries in the region were also used to provide a context for these two main sites.

Hydrological changes during the three-year field component were affected by the ending of a drought and then a major flood a year later as well as by ongoing anthropogenic flow reduction and augmentation. These influences on hydrology were associated with an initially high seagrass coverage that was substantially reduced and showed signs of recovery only in the system that was affected by lower inflows. Such influences and responses also changed seasonally but to a much lesser extent than the responses to stochastic climatic events.

Natural flows were intermittent and varied substantially between years. Flooding flows represented up to 89% of the long-term annual average flow. Water quality was broadly typical of the region, with the exception of low pH in some tributaries, especially those of Anglesea estuary. Anthropogenic changes to flow were most evident at times of low natural flows and resulted in longer and more frequent periods of zero inflow to Painkalac estuary and a continual base flow to Anglesea. This base flow, from ponds containing coal ash, neutralised waters flowing from upstream and increased conductivity, except at times of high natural flow.

A three-state conceptual model of the magnitude and variability of water levels, based largely on the degree of tidal influence was identified and quantitatively assessed for the two estuaries that were the main focus of the study. These states in turn had a large influence on the area and inundation of benthic habitat. Floods tended to open the mouths of estuaries, which then remained tidal given sufficient flow to overcome sedimentary processes at the mouths. Low and zero inflow was a precondition for closure of the mouths of the estuaries. When closed, differences in inflow resulted in different endpoints in salinity patterns. From an initial pattern similar to a classic ‘salt wedge’, Painkalac estuary, with reduced inflow, quickly destratified and gradually became more saline, at times hypersaline. Anglesea estuary, with augmented flow, tended to remain stratified for longer until becoming completely fresh, given a long enough period of closure.

Episodic changes in the water quality of the estuaries were associated with different components of the freshwater flow regimes. At high flows, fresh waters of low pH with a high metal load entered Anglesea estuary. Except during the largest flood, when the estuary was completely flushed, this water was neutralised at the halocline and resulting in precipitation of metals. High flows into Painkalac were associated with elevated concentrations of clay-sourced suspended solids. During a closed period, with zero flow, a release of sediment-bound nutrients triggered by anoxia was observed in Painkalac, followed by an algal bloom.

The large decline in seagrass extent that was observed in both estuaries was closely related to floods and the subsequent reductions in potential habitat associated with the tidal states that followed. Analysis of historical patterns of extent against rainfall records suggested that periods of drought and extended mouth closures were related to establishment and expansion of beds. This model was similar to that described for South African estuaries and contrasted with more-seasonal patterns reported for local marine embayments.

Rates of in situ decomposition of seagrass detritus showed a mix of seasonal and disturbance-driven patterns of change, depending on estuary. Variability of these rates on a scale of 100s of metres was typically not significant, but there were a few episodes that were highly significant. A negative correlation between decomposition rate and seagrass extent was also observed. A novel technique for assessing cellulose decomposition potential in sediment, adapted from soil science, proved to be a useful tool for estuarine research. Results from this component of the study highlighted both small-scale variability that was inconsistent through time, and also stable differences in decomposition potential between depths and estuaries that were consistent with differences in hydrological state and salinity.

Given the relative lack of knowledge about processes in intermittent estuaries, particularly those relating to changes in freshwater inflow, results from this study will be of value both locally and for similar systems elsewhere. Locally, it is likely that flow regimes to both Anglesea and Painkalac estuaries will be reduced, following closure of the mine power station at Anglesea and due to increased demand from the reservoir above Painkalac. There is potential to manage flows from each of these sources to minimise downstream effects. Regionally, and globally, there are many intermittent estuaries in areas with Mediterranean-type climates. It has been predicted that the climates of these regions will become drier but with an increase in intensity of storm events, both of which have ramifications for flow regimes to estuaries. It is hoped that results of this study will contribute to more informed management of intermittent estuaries in the context of these likely changes.

Interview with Sumbangan Baja

Dr Sumbangan Baja is an Indonesian who studied for a PhD in Australia at Sydney University on an Australian Development Scholarship, 1998-2001. He completed a Masters degree at Massey University in New Zealand, on a New Zealand Government scholarship. He met his wife in New Zealand - she was also an Indonesian student. The interview is conducted in English on 24 October 2014 by Dr Jemma Purdey of Deakin University and Dr Ahmad Suaedy of the Abdurrahman Wahid Centre for Inter-faith Dialogue and Peace at Universitas Indonesia. This set comprises: an interview recording, a photograph, and a timed summary.

Mitigation of N₂O emissions from surface-irrigated cropping systems using water management and the nitrification inhibitor DMPP

Soils under irrigated agriculture are a significant source of nitrous oxide (N2O) owing to high inputs of nitrogen (N) fertiliser and water. This study investigated the potential for N2O mitigation by manipulating the soil moisture deficit through irrigation scheduling in combination with, and in comparison to, using the nitrification inhibitor, 3,4-dimethylpyrazole phosphate (DMPP). Lysimeter cores planted with wheat were fitted with automated chambers for continuous measurements of N2O fluxes. Treatments included conventional irrigation (CONV), reduced deficit irrigation (RED), CONV-DMPP and RED-DMPP. The total seasonal volume of irrigation water applied was constant for all treatments but the timing and quantity in individual irrigation applications varied among treatments. 15N-labelled urea was used to track the source of N2O emissions and plant N uptake. The majority of N2O emissions occurred immediately after irrigations began on 1 September 2014. Applying RED and DMPP individually slightly decreased N2O emissions but when applied in combination (RED-DMPP) the greatest reductions in N2O emissions were observed. There was no effect of treatments on plant N uptake, 15N recovery or yield possibly because the system was not N limited. Half of the plant N and 53% to 87% of N2O was derived from non-fertiliser sources in soil, highlighting the opportunity to further exploit this valuable N pool.